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Publications

2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 |2010 | 2009 |2008 |2007 | 2006 | 2005-2001 | 2000-1996 | 1995-1990 |

 

2018

Non-invasive detection of urothelial cancer through the analysis of driver gene mutations and aneuploidy Springer S, Chen C-H, Del Carmen Rodriguez Pena M, Li L, Douville C, Wang Y, Cohen J, Taheri D, Afsari B, Silliman N, Schaefer J, Ptak J, Dobbyn L, Papoli M, Kinde I, Tregnago A C, Bezerra S M, VandenBussche C, Fujita K, Ertoy D, Cunha I W, Yu L, Bivalacqua T J, Grollman A P, Diaz Jr L A, Karchin R, Danilova L, Huang C-Y, Shun C-T, Turesky R J, Yun B H, Rosenquist T A, Pu Y-S , Hruban R, Tomasetti C, Papadopoulos N, Kinzler K W, Vogelstein B, DickmanK G, Netto G J. eLife, 2018. PMID: 29557778

2017

Epidemiology, diagnosis, preoperative evaluation and prognostic assessment of upper-tract urothelial carcinoma (UTUC). Soria F., Shariat S.F., Lerner S.P., Fritsche H-M., Rink M., Kassouf W., Spiess P.E., Lotan Y., Ye D., Fernandez M.I., Kikuchi E., Chade D.C., Babjuk M., Grollman A.P., Thalmann G.N. World J Urol 35(3):379-387,2017. PMID: 27604375

DNA deformation-coupled recognition of 8-oxoguanine: conformational kinetic gating in human DNA glycosylase. Li H., Endutkin A.V., Bergonzo C., Fu L., Grollman A.P., Zharkov D.O., Simmerling C. JACS 139(7):2682-2692, 2017. PMID: 28098999

Human liver-kidney model elucidates the mechanisms of aristolochic acid nephrotoxicity. Chang S-Y., Weber E.J., Sidorenko V.S., Chapron A., Yeung C.K., Gao C, Mao Q., Shen D., Wang J., Rosenquist T.A., Dickman K.G., Neumann T., Grollman A.P., Kelly E.J., Himmelfarb J., Eaton D.L. JCI Insights 2(22), 2017. PMID: 29202460

A Rapid Throughput Method To Extract DNA from Formalin-Fixed Paraffin- Embedded Tissues for Biomonitoring Carcinogenic DNA Adducts. Yun BH, Xiao S, Yao L, Krishnamachari S, Rosenquist TA, Dickman KG, Grollman AP, Murugan P, Weight CJ, Turesky RJ. Chem Res Toxicol 30(12):2130-2139, 2017. PMID: 29120619

2016

Low-Coverage Exome Sequencing Screen in Formalin-Fixed Paraffin-Embedded Tumors Reveals Evidence of Exposure to Carcinogenic Aristolochic Acid. Castells X, Karanović S, Ardin M, Tomić K, Xylinas E, Durand G, Villar S, Forey N, Le Calvez-Kelm F, Voegele C, Karlović K, Mišić M, Dittrich D, Dolgalev I, McKay J, Shariat SF, Sidorenko VS, Fernandes A, Heguy A, Dickman KG, Olivier M, Grollman AP, Jelaković B, Zavadil J. Cancer Epidemiol Biomarkers Prev.; 24 (12): 1873-81 (2015) PMID: 26383547

Marcus D.M., Grollman A.P. Toxicity of Botanical Medicines: An Overlooked Global Health Problem. AM J Public Health. 106(1):16-7 (2016). PMID: 26562106

Li H., Endutkin A.V., Bergonzo C., Campbell A.J., de Los Santos C., Grollman A., Zharkov D.O., Simmerling C. A dynamic checkpoint in oxidative lesion discrimination by formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 44(2):683-94 (2016). PMID: 26553802

Gao S., Honey S., Futcher B., Grollman A.P. The non-homologous end-joining pathway of S. cerevisiae works effectively in G1-phase cells, and relegates cognate ends correctly and non-randomly. DNA Repair 42:1-10 (2016). PMID: 27130982

Grollman A.P., Marcus D.M. Global hazards of herbal remedies: lessons from Aristolochia. EMBO 17(5):619-625(2016). PMID: 27113747

Guo J., Yun B.H., Upadhyaya P., Yao L., Krishnamachari S., Rosenquist T.A., Grollman A.P., Turesky R.J. Multiclass Carcinogeneic DNA Adduct Quantification in formalin-fixed Paraffin-Embedded Tissues by Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry. Anal Chem 88(9):4780-4787(2016). PMID: 27043225

Chen C.H., Dickman K.G., Huang C.Y., Shun C.T., Tai H.C. Huang K.H., Wang S.M., Lee Y.J., Grollman A.P., Pu Y.S. Recurrence pattern and TP53 mutation in upper urinary tract urothelial carcinoma. Oncotarget (2016, Jun), [Epub ahead of print]. PMID: 27286260

Rosenquist T.A., Grollman A.P. Mutational signature of aristolochic acid: Clue to the recognition of a global disease. DNA Repair 44:205-211(2016). PMID: 27237586

Hashimoto K., Zaitseva I.N., Bonala R., Attaluri S., Ozga K., Iden C.R., Johnson F., Moriya M., Grollman A.P., Sidorenko V.S. Sulfotransferase-1A1-dependent bioactivation of aristolochic acid I and N-hydroxyaristolactam I in human cells. Carcinogenesis 37(7):647-55 (2016). PMID: 27207664

Hoang M.L., Kinde I., Tomasetti C., McMahon K.W., Rosenquist T.A., Grollman A.P., Kinzler K.W., Vogelstein B., Papadopoulos N. Genome-wide quantification of rare somatic mutations in normal human tissues using massively parallel sequencing. Proc Natl Acad Sci, 113(35):9846-9851, (2016). PMID: 27528664

Hoang M.L., Chen C.H., Chen P-C., Roberts N.J., Dickman K.G., Yun B.H., Turesky R.J., Pu Y-S., Vogelstein B., Papadopoulos N., Grollman A.P., Kinzler K.W., Rosenquist T.A. Aristolochic Acid in the etiology of renal cell carcinoma. Cancer Epidemiol Biomarkers Prev, 113(35):9846-9851, (2016). PMID: 27555084

Soria F., Shariat S.F., Lerner S.P., Fritsche H-M., Rink M., Kassouf W., Spiess P.E., Lotan Y., Ye D., Fernandez M.I., Kikuchi E., Chade D.C., Babjuk M., Grollman A.P., Thalmann G.N. Epidemiology, diagnosis, preoperative evaluation and prognostic assessment of upper-tract urothelial carcinoma (UTUC). World J Urol, (2016), Sept, [Epub ahead of print]. PMID: 27604375

Hashimoto K., Bonala R., Johnsona F., Grollman A.P., Moriya M. Y-family DNA polymerase-independent gap-filling translesionsynthesis across aristolochic acid-derived adenine adducts in mouse cells. DNA Repair 46:55-60 (2016). PMID: 27497692

2015
Hashimoto, K., Wada, K., Matsumoto, K. and Moriya, M., Physical interaction between SLX4 (FANCP) and XPF (FANCQ) proteins and biological consequences of interaction-defective missense mutations, DNA Repair 35, 48-54, 2015. PMID: 26453996

Dickman K.G., Fritsche H-M., Grollman A.P., Thalmann G., Catto J. Epidemiology and Risk Factors for Upper Urinary Urothelial Cancers. In (Chapter 1): Upper Tract Urothelial Carcinoma, Shariat S.F., Xylinas E. Springer Science & Business Media NY (2015)

Jelaković B., Lela I.V., Karanović S., Dika Z, Kos J., Dickman K., Šekoranja M., Poljičanin T., Mišic M., Premuzić V., Abramović M., Matijević V., Medved M.M., Cvitković A., Edwards K., Fuček M., Leko N., Teskera T., Laganović M., Čvorišćec D., and Grollman A.P. Chronic dietary exposure to Aristolochic Acid and kidney function in native farmers from a Croatian endemic area and Bosnian immigrants. Clin J Am Soc Nephro 10:(2015) – ahead of print

Kuznetsov N.A., Bergonzo C., Campbell A.J., Li H., Mechetin G.V., de los Santos C., Grollman A.P., Fedorova O.S., Zharkov K.O., Simmerling C. Active destabilization of base pairs by a DNA glycosylase wedge initiates damage recognition.

Nucleic Acids Research 43(1): (2015) ahead of print

2014

Matin S.F., Shariat S.F., Milowsky M.I., Hansel D.E., Kassouf W., Koppie T., Bajorin D., Grollman A.P. Highlights from the first symposium on upper tract urothelial carcinoma. Urol Oncol, S1078-1439 (2014), PMID 24397995

Ivković V., Karanović S, Edwards K., Grollman A.P., Jelaković B. Is herbal tea consumption a factor in endemic nephropathy? Eur J Epidemiol, (2014), Published online March 13 2014, PMID 24623403

Sidorenko V., Attaluri S., Zaitseva I., Iden C.R., Dickman K.G. Johnson F, Grollman A.P. Bioactivation of the human carcinogen aristolochic acid. Carcinogenesis, (2014)

Romanov V., Whyard T., Waltzer W.C., Grollman A.P., Rosenquist T. Aristolochic acid-induced apoptosis and G2 cell cycle arrest depends on ROS generation and MAP kinases activation. Arch Toxicol (2014)

Yun B.H., Yao L., Jelaković B., Nikolić J., Dickman K.G., Grollman A.P., Rosenquist T.A., Turesky R.J. Formalin-fixed paraffin-embedded tissue as a source for quantitation of carcinogen DNA adducts: aristolochic acid as a prototype carcinogen. Carcinogenesis (2014)

2013 Top

Jelaković B., Nikolić J., Radovanovic Z., Nortier J., Cosyns J.P., Grollman A.P., et al Consensus statement on screening, diagnosis, classification and treatment of endemic (Balkan) nephropathy. Nephrol Dial Transplant, 2013. PMID 24166461

Grollman A.P. Aristolochic acid nephropathy: Harbinger of a global iatrogenic disease.  Environmental and Molecular Mutagenesis, 54(1): 1-7, 2013. PMID23238808

Chen C-H, Dickman K G, Huang C-Y, Moriya M, Shun C-T, Tai H-C, Huang K-H, Wang S-M, Lee Y-J, Grollman A P, Pu Y-S. Aristolochic Acid-Induced Upper Tract Urothelial Carcinoma in Taiwan: Clinical Characteristics and Outcomes.   Int J Cancer, Early Edition 2013. PMID23292929

Killela P J, Reitman Z J, Jiao Y, Bettegowda C, Agrawal N, Diaz Jr. L A, Friedman A H, Friedman H, Gallia G L, Giovanella B C, Grollman A P, He T-C,
He Y, Hruban R H, Jallo G I, Mandahl N, Meeker A K, Mertens F, Netto G J, Rasheed B A, Riggins G J, Rosenquist T A, Schiffman M, Shih l-M, Theodorescu D, Torbenson M S, Velculescu V E, Wang T-L, Wentzensen N, Wood L D, Zhang M, McLendon R E, Bigner D D Kinzler K W, Vogelstein B, Papadopoulos N, Yan H. TERT promoter mutations occur frequently in gliomas and a subset of tumors derived from cells with low rates of self-renewal. PNAS, Early Edition 2013. PMID23530248

2012 Top

Chen C.H., Dickman K.G., Moriya M., Zavadil J., Sidorenko V.S., Edwards K.L., Gnatenko D.V., Wu L., Turesky R.J., Wu X.R., Pu Y.S., and Grollman A.P. Aristolochic acid-associated urothelial cancer in Taiwan. Proc Natl Acad Sci USA, 109(21):8241-6, 2012.  PMID22493262

Jelakovic B., Karanovic S., Vukolic-Lela I., Miller F., Edwards K., Nikolic J., Tomic K., Slade N., Brdar B., Turesky R., Stipancic Z., Dittrich D., Grollman A.P., and Dickman K.G. Aristolactam-DNA adducts are a biomarker of environmental exposure to aristolochic acid. Kidney Int 81:559-67, 2012.  PMID22071594

Lukin M., Zaliznyak T., Johnson F., and de Los Santos C. Structure and stability of DNA containing an aristolactam II-dA lesion: implications for the NER recognition of bulky adducts. Nucleic Acids Res 40:2759-70, 2012.  PMID22121223

Priestap H.A., Torres M.C., Rieger R.A., Dickman K.G., Freshwater T., Taft D.R., Barbieri M.A., and Iden C.R. Aristolochic acid I metabolism in the isolated perfused rat kidney. Chem Res Toxicol 25:130-9, 2012.  PMID22118289

Romanov V., Sidorenko V., Rosenquist T.A., Whyard T., and Grollman A.P. A fluorescence-based analysis of aristolochic acid-derived DNA adducts. Anal Biochem 427:49-51, 2012.  PMID22484040

Sidorenko V.S., Yeo J.E., Bonala R.R., Johnson F., Scharer O.D., and Grollman A.P. Lack of recognition by global-genome nucleotide excision repair accounts for the high mutagenicity and persistence of aristolactam-DNA adducts. Nucleic Acids Res 40:2494-505, 2012.  PMID22121226

Yun B., Rosenquist T., Sidorenko V., Iden C.R., Chen C.H., Pu Y.S., Bonala R., Johnson F., Dickman K.G., Grollman A.P., and Turesky R.J. Biomonitoring of human AL-DNA adducts in human tissues using ultra-performance liquid chromatography/ion-trap mass spectroscopy. Chem Res Toxicol 25:1119-31, 2012.  PMID22515372

Hashimoto, K., Cho, Y., Yang, I.-Y., Akagi, J., Ohashi, E., Tateishi, S., de Wind, N., Hanaoka, F., Ohmori, H., and Moriya, M. The vital role of pol z and REV1 in mutagenic, but not correct, DNA synthesis across  benzo[a]pyrene-dG and the recruitment of pol z by REV1 to a replication-stalled site. J. Biol. Chem. 287, 9613-9622, 2012. PMID22303021

2011 Top

Dickman K.G., Sweet D.H., Bonala R., Ray T., and Wu A. Physiological and molecular characterization of aristolochic acid transport by the kidney. J Pharmacol Exp Ther 338:588-97, 2011.  PMID21546538

Moriya M., Slade N., Brdar B., Medverec Z., Tomic K., Jelakovic B., Wu L., Truong S., Fernandes A., and Grollman A.P. TP53 mutational signature for aristolochic acid: an environmental carcinogen. Int J Cancer 129:1532-6, 2011.  PMID21413016

Romanov V., Whyard T., Bonala R., Johnson F., and Grollman A. Glutamate dehydrogenase requirement for apoptosis induced by aristolochic acid in renal tubular epithelial cells. Apoptosis 16:1217-28, 2011.  PMID21901531

Rosenquist T.A. Genetic loci that affect aristolochic acid-induced nephrotoxicity in the mouse. Am J Physiol Renal Physiol 300:F1360-7, 2011.  PMID21429970

Bergonzo, C., Campbell, A.J., de los Santos, C., Grollman, A.P., Simmering, C. Energetic preference of 8-oxoG: eversion pathways in a DNA glycosylase. J Am chem. Soc. 133:14504-14506, 2011.  PMID21848286

Yuan, B., You, C., Andersen, N., Jiang, Y., Moriya, M., O’Connor, T.R. and Wang, Y., The roles of DNA polymerases κ and ι in the error-free bypass of N2-carboxyalkyl-2'-deoxyguanosine lesions in mammalian cells. J. Biol. Chem. 286, 17503-17511, 2011.  PMID:21454642

2010 Top

Attaluri S., Bonala R.R., Yang I.Y., Lukin M.A., Wen Y., Grollman A.P., Moriya M., Iden C.R., and Johnson F. DNA adducts of aristolochic acid II: total synthesis and site-specific mutagenesis studies in mammalian cells. Nucleic Acids Res 38:339-52, 2010.  PMID19854934

Rosenquist T.A., Einolf H.J., Dickman K.G., Wang L., Smith A., and Grollman A.P. Cytochrome P450 1A2 detoxicates aristolochic acid in the mouse. Drug Metab Dispos 38:761-8, 2010.  PMID20164109

Shibutani S., Bonala R.R., Rosenquist T., Rieger R., Suzuki N., Johnson F., Miller F., and Grollman A.P. Detoxification of aristolochic acid I by O-demethylation: less nephrotoxicity and genotoxicity of aristolochic acid Ia in rodents. Int J Cancer 127:1021-7, 2010.  PMID20039324

Dickman, K.G. and Grollman, A.P. Nephrotoxicity of natural products: aristolochic acid and fungal toxins. Comprehensive Toxicology v7, Renal Toxicology (2nd ed), RG Schnellmann Seroes Ed., Elsevier: Oxford 433-458, 2010.  DOI:10.1016/s1872-0854(09)00007-1

2009 Top

Grollman A.P., Scarborough J., and Jelakovic B. Aristolochic Acid Nephropathy: An Environmental and Iatrogenic Disease, in Adv Mol Toxicol 3, J Fishbein, Ed., Elsevier:Amsterdam, 2009.  DOI: 10.1016/S1872-0854(09)00007-1

Sidorenko, V.S., Grollman, A.P., Jaruga, P., Dizdaroglu, M., Zharkov, D.O. Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase. FEBS J. 276(18): 5149-62, 2009.  PMID19674107

Song, K., Campbell, A.J., Bergonzo, C., de los Santos, C., Grollman, A.P., Simmerling, C. An improved reaction coordinate for nucleic acid base flipping studies. J Chem Theory Comput. 5(11):3105-3113, 2009.  DOI: 10.1021/ct9001575

Yang, I.-Y., Hashimoto, K., de Wind, N., Blair, I.A. and Moriya, M., Two distinct translesion synthesis pathways across a lipid peroxidation-derived DNA adduct in mammalian cells, J. Biol. Chem. 284, 191-198, 2009.  PMID:18981176

2008 Top

Song, D. Hornak, V., de los Santos, C., Grollman, A.P., Simmerling, Molecular mechanics parameters for the FapydG DNA lesion. C. J comput Chem. 29(1):17-23, 2008.  PMID17551974

2007 Top

Marcus, D.M., Grollman, A.P. Limitations of “evidence-based indications” for herbs. Mayo Clin Proc. 82(11): 1433, 2007.  PMID17976363

Song K., Kelso C., de los Santos C., Grollman A.P. & Simmerling C. Molecular simulations reveal a common binding mode for glycosylase binding of oxidatively damaged DNA lesions. J Am Chem Soc. 2007 129, 14536-14537.    PMID17988127
 
Grollman AP, Shibutani S, Moriya M, Miller F, Wu L, Moll U, Suzuki N, Fernandes A, Rosenquist T, Medverec Z, Jakovina K, Brdar B, Slade N, Turesky RJ, Goodenough AK, Rieger R, Vukelić M, Jelaković B.   Aristolochic acid and the etiology of endemic (Balkan) nephropathy. Proc Natl Acad Sci U S A. 2007 104(29):12129-34.  PMID17620607
 
Grollman AP, Jelaković B. Role of environmental toxins in endemic (Balkan) nephropathy. J Am Soc Nephrol. 2007 18(11):2817-23.  PMID17942951
 
Blumenberg M, Gao S, Dickman K, Grollman AP, Bottinger EP, Zavadil J. Chromatin structure regulation in transforming growth factor-beta-directed epithelial-mesenchymal transition. Cells Tissues Organs. 2007;185(1-3):162-74.  PMID17587822

Shibutani S, Dong H, Suzuki N, Ueda S, Miller F, Grollman AP.   Selective toxicity of aristolochic acids I and II. Drug Metab Dispos. 2007 35(7):1217-22.   PMID17392392

 
2006 Top

Marcus, D.M., Grollman, A.P. Science and government: Review for NCCAM is overdue. Science 313(5785): 301-2, 2006.  PMID16857923

Morrissey, P.E., Gautam, A., Yang, A., Grollman A.P., Esparza, A., Gohh, R.Y., Monaco, A.P. Transplantation for Chinese herb nephropathy. Clin Transpl. 560, 2006.  PMID18365433

Dong, F., Suzuki, N., Torres, M. C., Bonala, R. R., Johnson, F., Grollman, A. P., and Shibutani, S. Quantitative determination of aristolochic acid-derived DNA adducts in rats using 32P-postlabeling/polyacrylamide gel electrophoresis analysis. Drug Metab. Dispos. 34,1122-1127 (2006).  PMID16611860

Rieger, R. A.; Zaika, E. I.; Xie, W.; Johnson, F.; Grollman, A. P.; Iden, C. R.; Zharkov, D. O. Proteomic Approach to Identification of Proteins Reactive for Abasic Sites in DNA, Mol. Cell Proteomics, 2006, , 858-867.  PMID16474175
 
Stein S., Lao Y., Yang I.-Y., +Hecht S.S., and Moriya M., Genotoxicity of acetaldehyde- and crotonaldehyde-induced 1,N2-propanodeoxyguanosine DNA adducts in human cells, Mutat. Res. 2006, 608: 1-7.  PMID16797223

Liu, X., Lao, Y., Yang, I.-Y., Hecht, S.S., and Moriya, M., Replication-coupled repair of crotonaldehyde/acetaldehyde-induced guanine-guanine interstrand cross-links and their mutagenicity, Biochemistry 2006, 45: 12898-129056.  PMID17042508
 
Kalam, M.A., Haraguchi, K., Chandani, S., Loechler, E.L., Moriya, M., Greenberg, M.M. and Basu, A.K., Genetic effects of oxidative DNA damages: comparative mutagenesis of the imidazole ring-opened formamidopyrimidines (Fapy lesions) and 8-oxo-purines in simian kidney cells, Nucleic Acids Res. 2006, 34: 2305-2315.  PMID16679449

Song, K., Hornak, V., de los Santos, C., Grollman, A. & Simmerling, C. Computational analysis of the binding mode of 8-oxo-guanine to formamidopyrimidine-DNA glycosylase Biochemistry 2006, 45: 10886-10894.  PMID16953574

Liu X, Lao Y, Yang IY, Hecht SS, Moriya M. Replication-coupled repair of crotonaldehyde/acetaldehyde-induced guanine-guanine interstrand cross-links and their mutagenicity. Biochemistry. 2006 45(42):12898-905.  PMID17042508

Pollack M, Yang IY, Kim HY, Blair IA, Moriya M. Translesion DNA Synthesis across the heptanone--etheno-2'-deoxycytidine adduct in cells. Chem Res Toxicol. 2006 19(8):1074-9.  PMID16918247
 
Kropachev KY, Zharkov DO, Grollman AP. Catalytic mechanism of Escherichia coli endonuclease VIII: roles of the intercalation loop and the zinc finger. Biochemistry. 2006 45(39):12039-49.  PMID17002303

Golan G, Zharkov DO, Grollman AP, Dodson ML, McCullough AK, Lloyd RS, Shoham G. Structure of T4 pyrimidine dimer glycosylase in a reduced imine covalent complex with abasic site-containing DNA. J Mol Biol. 2006 362(2):241-58.   PMID16916523
 
Gening LV, Klincheva SA, Reshetnjak A, Grollman AP, Miller H. RNA aptamers selected against DNA polymerase beta inhibit the polymerase activities of DNA polymerases beta and kappa. Nucleic Acids Res. 2006 34(9):2579-86.   PMID16707660
 

2005-2001 Top

Tan X, Bonala RR, Suzuki N, Johnson F, Grollman AP, Shibutani S. Mutagenic specificity of 2-acetylaminonaphthalene-derived DNA adduct in mammalian cells. Chem Biol Interact 2005; 152: 131-138.
 

Shibutani, S., Kim, S. Y., and Suzuki, N. 32P-Postlabeling DNA damage assay: PAGE, TLC and HPLC. In: DNA Repair Protocols: Eukaryotic systems, second edition. (Ed.) Daryl Henderson. Humana Press Inc. New Jersey, USA.. (2005)
 
Tan, X., Bonala, R.-R., Suzuki, N., Johnson, F., Grollman, A. P., and Shibutani, S. Mutagenic specificity of 2-acetylaminonaphthalene-derived DNA adduct in mammalian cells. Chem.-Biol. Interact. 152, 131-138 (2005).
 

Hranjec, T., Kovac, A., Kos, J., Mao, W., Chen, J. J., Grollman, A. P., and Jelakovic, B. Endemic nephropathy: the case for chronic poisoning by aristolochia. Croat. Med. J. 46, 116-125 (2005)
 

Cheng, X., Hornak, V., de los Santos, C., Grollman, A. P. & Simmerling, C. Dynamic behavior of DNA base pairs containing 8-oxoguanine. J. Am. Chem. Soc. 127: 13906-13918 (2005).
 

Conlon KA, Miller H, Rosenquist TA, Zharkov DO, Berrios M. The murine DNA glycosylase NEIL2 (mNEIL2) and human DNA polymerase beta bind microtubules in situ and in vitro. DNA Repair  4: 419-31 (2005).
 

Zharkov DO, Grollman AP. The DNA trackwalkers: principles of lesion search and recognition by DNA glycosylases. Mutat Res 33: 5006-16 (2005).
 

Golan G, Zharkov DO, Feinberg H, Fernandes AS, Zaika EI, Kycia JH, Grollman AP, Shoham G.   Structure of the uncomplexed DNA repair enzyme endonuclease VIII indicates significant interdomain flexibility. Nucleic Acids Res. 33(15):5006-16 (2005)
 

Grollman AP. Academic perspectives on dietary supplements use: the need for new guidelines. Thromb Res. 117(1-2):185-92 (2005)
 

Grollman AP, Simmerling C. Dynamic behavior of DNA base pairs containing 8-oxoguanine. J Am Chem Soc. 127(40):13906-18 (2005)

 
Kalam, M.A., Haraguchi, K., Alimchandani, S., Loechler, E.L., Moriya, M., Greenberg, M.M. and Basu, A.K., Comparative mutagenesis of Fapy•dG and 8-oxo-dG in mammalian cells, Biochemistry, submitted, 2004.


Suzuki, N., Itoh, I., Poon, K., Masutani, C., Hanaoka, F., Ohmori, H., Yoshizawa, I., and Shibutani, S. Translesion synthesis past estrogen-derived DNA adducts by human DNA polymerases. Biochemistry 43. 6304-6311 (2004).

Kim, S. Y., Suzuki, N., Y. R. Santosh Laxmi, and Shibutani, S. Genotoxic mechanism of tamoxifen in developing endometrial cancer. Drug Metab. Rev. 36. 199-218 (2004).

Y. R. Santosh Laxmi, Suzuki, N., Kim, S. Y., and Shibutani, S. Synthesis of oligodeoxynucleotides containing a diastereoisomer of alpha-(N2-2’-deoxyguanosinyl)-N-desmethyltamoxifen. Bioorg. Med. Chem. Lett. 14. 4051-4054 (2004).

Suzuki, N., Prabhu, M. P., and Shibutani, S. Detection of DNA adducts by 32P-postlabeling analyses. In: Optimization in Drug Discovery: In-vitro Methods, Yan, Z., and Caldwell, G. W. (Eds). Humana Press Inc. New Jersey, USA (2004) Humana Press Inc.

Miller H, Fernandes A, Zaika E, McTigue M, Torres C, Wente M, Iden C, Grollman A.P. Stereoselective excision of thymine glycol from oxidatively damaged DNA. Nucleic Acids Research. 2004; 32, No. 1: 338-45

Gola G, Zharkov D.O., Fernandes A.S., Zaika E, Kycia J.H., Wawrzak Z, Grollman A.P., Shoham G. Crystallization and preliminary crystallographic analysis of endonuclease VIII in its uncomplexed form. Acta Crystallographics, 2004, D60, 0 (in press)

Freisinger E, Grollman A.P., Miller H, Kisker, C. Lesion (in)tolerance reveals insights into DNA replication fidelity. EMBO, 2004; 23: 1494 -1505

Zaika E. I., Perlow R.A., Matz E, Broyde S, Gilboa R, Grollman A.P., Zharkov D.O. DNA: Replication, Repair and Recombination. J Biol Chem. 2004; 279: 4849 – 61

Polosina Y.Y., Rosenquist T.A., Grollman A.P., Miller H. “Knock down” of DNA polymerase ß by RNA interference: recapitulation of null phenotype. DNA Repair. 2004; (in press)

Gao S., Chen J., Brodsky S.V., Huang H., Adler S., Lee J.H., Dhadwal N., Cohen-Gould L., Gross S.S., Goligorsky M.S. Docking of endothelial nitric oxide synthase (eNOS) to the mitochondrial outer membrane: a pentabasic amino acid sequence in the autoinhibitory domain of eNOS targets a proteinase K-cleavable peptide on the cytoplasmic face of mitochondria. J Biol Chem. 2004; 279(16):15968-74.

Minetti, C.A.S.A., Remeta, D.P., Zharkov, D.O., Plum, G.E., Johnson, F., Grollman, A.P. and Breslauer, K.J. Energetics of Lesion Recognition by DNA Repair Protein: Thermodynamic Characterization of Formamidopyrimidine-glycosylase (Fpg) Interactions with Damaged DNA Duplexes. J. Mol. Biol. 2003; 328: 1047-1060

Zharkov, D., Shoham, G. and Grollman, A.P. Structural characterization of the Fpg family of DNA glycosylases.
DNA Repair. 2003; 2: 839 - 862

Freisinger, E., Fernandes, A., Grollman, A.P., and Kisker, C. Crystallographic Characterization of an Exocyclic DNA Adduct: 3, N4-etheno-2’-deoxyctydine in the Dodecamer 5’-CGCGAATT?CGCG-3’ J. Mol. Biol. 2003; 329: 685-697.

Miller H, Grollman AP. DNA repair investigations using siRNA. DNA Repair (Amst). 2003 Jun 11;2(6):759-63.

Rosenquist TA, Zaika E, Fernandes AS, Zharkov DO, Miller H, Grollman AP. The novel DNA glycosylase, NEIL1, protects mammalian cells from radiation-mediated cell death. DNA Repair (Amst). 2003 May 13;2(5):581-91.

Krahn JM, Beard WA, Miller H, Grollman AP, Wilson SH. Structure of DNA polymerase Beta with the mutagenic DNA lesion 8-oxodeoxyguanine reveals structural insights into its coding potential. Structure (Camb). 2003 Jan;11(1):121-7.

Yang IY, Miller H, Wang Z, Frank EG, Ohmori H, Hanaoka F, Moriya M. Mammalian translesion DNA synthesis across an acrolein-derived deoxyguanosine adduct. Participation of DNA polymerase eta in error-prone synthesis in human cells. J Biol Chem. 2003 Apr 18;278(16):13989-94.

Yasui, M., Matsui, S., Y.R. Santosh Laxmi, Suzuki, N., Kim, S. Y., Shibutani, S. and Matsuda, T. Mutagenic events induced by 4-hydroxyequilin in supF shuttle vector plasmid propagated in human cells. Carcinogenesis 24, 911-917 (2003).

Shibutani, S., Suzuki, N., Y. R. Santosh Laxmi, Schild, L. J., Divi, R. L., Grollman, A. P., and Poirier, M. C. Identification of tamoxifen-DNA adducts in monkeys treated with tamoxifen. Cancer Res. 63, 4402 - 4406 (2003).

Kim, S. Y., Suzuki, N., Y. R. Santosh Laxmi, Rieger, R., and Shibutani, S. alpha-Hydroxylation of tamoxifen and toremifene by human and rat cytochrome P450 3A family enzymes. Chem. Res. Toxicol. 16. 1138-1144 (2003).

Sanchez, A.M., Minko, I.G., Kurtz, A.J., Kanuri, M., Moriya, M. and Lloyd, R.S., Comparative evaluation of the bioreactivity and mutagenic spectra of acrolein-derived alpha-HOPdG and gamma-HOPdG regioisomeric deoxyguanosine adducts, Chem. Res. Toxicol. 16: 1019-1028. (2003)

Tan, X., Suzuki, N., Grollman, A. P., and Shibutani, S. Mutagenic events in Escherichia coli and mammalian cells in response to acetylaminofluorene-derived DNA adducts positioned in the Nar I restriction enzyme site. Biochemistry 41, 14255-14262 (2002).

Yang IY, Chan G, Miller H, Huang Y, Torres MC, Johnson F, Moriya M. Mutagenesis by acrolein-derived propanodeoxyguanosine adducts in human cells. Biochemistry. 2002 Nov 19;41(46):13826-32.

Shibutani, S., Suzuki, N. and Grollman, A.P. Tamoxifen-DNA adducts: biomarkers for drug-induced endometrial cancer in Biomarkers of environmentally associated disease: technology, concepts and perspectives, ed by S.H. Wilson and W.A. Suk. CRC Press LLC, New York, 2002 pp 127-137

Zharkov, D.O.and Grollman A.P. Combining structural and bioinformatics methods for the analysis of functionally important residues in DNA glycosylases. Free Radic Biol. Med. 32:1254-63 (2002).

Suzuki, N., Ohashi, E., Kolbanovskiy, A., Geacintov, N.E., Grollman, A.P., Ohmori, H., and Shibutani, S. Translesion synthesis by human DNA polymerase kappa on a DNA template containing a single stereoisomer of dG-(+)- or dG-(-)-anti-N(2)-BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene). Biochemistry 41:6100-6 (2002)

Gilboa, R., Zharkov, D.O., Golan, G., Fernandes, A.S., Gerchman, S.E,, Matz, E., Kycia, J.H., Grollman, A.P., and Shoham, G. Structure of formamidopyrimidine-DNA glycosylase covalently complexed to DNA. J. Biol. Chem. 277:19811-6 (2002).

Yang, I.Y., Johnson, F., Grollman, A.P., and Moriya, M. Genotoxic mechanism for the major acrolein-derived deoxyguanosine adduct in human cells. Chem Res Toxicol. 15:160-4 (2002).

Zharkov, D.O., Golan, G., Gilboa, R., Fernandes, A.S., Gerchman, S.E., Kycia, J.H., Rieger, R.A., Grollman, A.P., and Shoham, G. Structural analysis of an Escherichia coli endonuclease VIII covalent reaction intermediate. EMBO J. 21:789-800 (2002).

Terashima I, Suzuki N, Shibutani S. 32P-Postlabeling/polyacrylamide gel electrophoresis analysis: application to the detection of DNA adducts. Chem. Res. Toxicol. 15:305-11 (2002).

Santosh Laxmi YR, Suzuki N, Dasaradhi L, Johnson F, Shibutani S. Preparation of oligodeoxynucleotides containing a diastereoisomer of alpha-(N(2)-2'-deoxyguanosinyl)tamoxifen by phosphoramidite chemical synthesis. Chem. Res. Toxicol.;15:218-25 (2002).

Shibutani, S., Suzuki, N., Tan, X., Johnson, F., and Grollman, A.P. Influence of flanking sequence context on the mutagenicity of acetylaminofluorene-derived DNA Adducts in mammalian cells. Biochemistry 40(12); 3717-3722 (2001).

Yang, I-Y, Hossain, M., Miller, H., Khullar, S., Johnson, F., Grollman, A.P., and Moriya, M. Responses to the major acrolein-derived deoxyguanosine adduct in Escherichia coli. J. Biol. Chem. 276, 9071-9076 (2001).

Levine, R.L., Miller, H., Grollman, A.P., Ohashi, E., Ohmori, H., Masutani, C., Hanoaka, F., and Moriya, M. Translesion DNA synthesis catalyzed by human pol h and pol k across 1,N6-ethenodeoxyadenosine. J. Biol. Chem. 276, 18717-18721 (2001)

Shibutani, S., Ravindernath, A., Terashima, I., Suzuki, N., Santosh Laxmi, Y.R., Kanno, Y., Suzuki, M., Idil Apak, T., Sheng, J.J., and Duffel, M.W. Mechanism of lower genotoxicity of toremifene compared with tamoxifen. Cancer Res. 61, 3925-3931 (2001)

Yasui, M., Matsui, S., Ihara, M., Santosh Laxmi, Y.R., Shibutani, S., and Matsuda, T. Translesional synthesis on a DNA template containing N2-methyl-2'-deoxyguanosine catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I. Nucleic Acids Res. 29, 1994-2001 (2001)

Terashima, I., Matsuda, T., Fan, T-W., Suzuki, N., Kobayashi, J., Kohda, K., and Shibutani, S. Miscoding potential of the N2-ethyl-2'-deoxyguanosine DNA adduct by the exonuclease-free klenow fragment of Escherichia coli DNA polymerase I. Biochemistry 40, 4106-4114 (2001)

Terashima, I., Suzuki, N., and Shibutani, S. Mutagenic properties of estrogen-quinone-derived DNA adducts in simian kidney cells. Biochemistry 40, 166-172 (2001)

2000-1996 Top

Shibutani, S., Suzuki, N. and Grollman, A.P. Tamoxifen-DNA adducts: Biomarkers for drug-induced endometrial cancer. In: Progress in Nucleic Acid Research & Molecular Biology, Moldave, K. (Ed.), Academic Press, in press (2000).

Pandya, G.A., Yang, I-Y, Grollman, A.P., and Moriya, M. Escherichia coli responses to a single DNA adduct. J. Bacteriology 182, 6598-6604 (2000).PDF (272Kb)

Zharkov, D.O., Gilboa, R., Yagil, I., Kycil, J.H., Gerchman, S.E., Shoham, G., and Grollman, A.P. A role for lysine 142 in the excision of adenine from A:G mispairs by MutY DNA glycosylase of Escherichia coli. Biochemistry 39, 14768-14778 (2000).

Levine, R.L., Yang, I.Y., Hossain, M., Pandya, G.A., Grollman, A.P. and Moriya, M. Mutagenesis induced by a single 1,N6-ethenodeoxyadenosine adduct in human cells. Cancer Res. 60, 4098-4104 (2000).PDF(264Kb)

Umemoto, A., Monden, Y., Suwa, M., Kanno, Y., Suzuki, M., Lin, C-X., Ueyama, Y., Abdul Momem, Md., Ravindernath, A., Shibutani, S., and Komaki, K. Identification of hepatic tamoxifen-DNA adducts in mice: a-(N2-deoxyguanosinyl)tamoxfen and a-(N2-deoxyguanosinyl)tamoxifen N-oxide. Carcinogenesis 21, 1737-1744 (2000)

Shibutani, S., Reardon, J.T., Suzuki, N., and Sancar. A. Excision of tamoxifen-DNA adducts by the human nucleotide excision repair systems. Cancer Res. 60, 2607-2610 (2000).

Zharkov, D.O., Rosenquist, T.A., Gerchman, S.E., and Grollman, A.P. Substrate specificity and reaction mechanisms of murine 8-oxoguanine-DNA glycosylases. J. Biol. Chem. 275, 28607-28617 (2000).

Shibutani, S., Ravindernath, A., Suzuki, N., Terashima, I., Sugarman, S.M., Grollman, A.P., and Pearl, M.L. Identification of tamoxifen-DNA adducts in the endometrium of women treated with tamoxifen. Carcinogenesis 21, 1461-1467 (2000).

Rieger, R.A., McTigue, M.M., Kycia, J.H., Gerchman, S.E., Grollman, A.P., and Iden, C.R. Characterization of a cross-linked DNA-endonuclease VIII repair complex by electrospray ionization mass spectrometry. J. Am. Soc. Mass Spectrometry 11, 505-515 (2000).

Kitagawa, M., Ranindernath, A., Suzuki, N., Rieger, R., Terashima, I., Umemoto, A., and Shibutani, S. Identification of tamoxifen-DNA adducts induced by a-acetoxy-N-desmethyltamoxifen. Chem. Res. Toxicol. 13, 761-769 (2000)

Conlon, K.A., Grollman, A.P., and Berrios, M. Immunolocalization of 8-oxoguanine in nutrient-deprived mammalian tissue culture cells. J. Histotechnology 23, 37-44 (2000).

Shibutani, S., Reardon, J.T., Suzuki, N., and Sancar, A. Exciswioni of tamoxifen-DNA adducts by the human nucleotide excision repair system. Cancer Res. 60, 2607-2610 (2000)

Miller, H., Prasad, R., Wilson, S.H., Johnson, F. and Grollman, A.P. 8-OxoGTP incorporation by DNA polymerase beta is modified by active-site residue Asn279. Biochemistry 39, 1029-1033 (2000).

Moriya, M., Pandya, G.A., Johnson, F. and Grollman, A.P. Cellular response to exocyclic DNA adducts. In: Exocyclic DNA Adducts in Mutagenesis & Carcinogenesis. Singer, B. & Bartsch, H. (Eds.) IARC Scientific Publication (Lyon) 150, pp. 263-270 (1999).

Umemoto, A., Monden, Y., Komaki, K., Suwu, M., Kanno, Y., Suzuki, M., Lin, C.X., Ueyama, Y., Momen, M.A., Ravindernath, A., and Shibutani, S. Tamoxifen-DNA adducts formed by a-acetoxytamoxifen N-oxide. Chemical Research in Toxicology 12, 1083-1089 (1999).

Tan, X., Suzuki, N., Grollman, A.P. and Shibutani, S. Comparison of the mutagenic properties of 8-oxo-7,8-dihydro-2'-deoxyadenosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine DNA lesions in mammalian cells. Carcinogenesis 20, 2287-2292 (1999).

Matsuda, T., Terashima, I., Matsumoto, Y., Yabushita, H., Matsui, S., and Shibutani, S. Effective utilization of N2-ethyl-2'-deoxyguanosine triphosphate during DNA synthesis catalyzed by mammalian replicative DNA polymerases. Biochemistry 38, 929-935 (1999).

Terashima, I, Suzuki, N., and Shibutani, S. Mutagenic potential of a-N2-deoxyguanosinyl) tamoxifen lesions, the major DNA adducts detected in endometrial tissues of patients treated with tamoxifen. Cancer Res. 59, 2091-2095 (1999).

Shibutani, S., Fernandes, A., Suzuki, N., Zhou, L., Johnson, F. and Grollman, A.P. Mutagenesis of the N-(Deoxyguanosin-8-yl)-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine DNA adduct in mammalian cells. J. Biol. Chem. 274, 27433-27438 (1999).

Bonala, R.R., Rieger, R.A., Shibutani, S., Grollman, A.P., Iden, C.R. and Johnson, F. 3,N4-Ethano-2'-deoxycytidine: Chemistry of incorporation into oligomeric DNA and reassessment of miscoding potential. Nucleic Acids Res. 27, 4725-4733 (1999).

Grollman, A.P. and Zharkov, D.O. Recognition and excision of bases from oxidatively damaged DNA by Fpg, Ogg1, and MutY proteins. In: Advances in DNA Damage and Repair. Oxygen Radical Effects, Cellular Protection and Biological Consequences. Dizdaroglu, M. and Karakaya, A.E. (Eds.), Plenum Publishers, NY, pp. 136-148 (1999).

Shibutani, S., Suzuki, N., Terashima, I., Sugarman, S.M., Grollman, A.P. and Pearl, M.L. Tamoxifen-DNA adducts detected in endometrium obtained from patients treated with tamoxifen. Chem. Res. Toxicol. 12, 646-653 (1999).

Deng, Y., Glimm, J., Wang, Y., Korobka, A., Eisenberg, M. and Grollman, A.P. Prediction of protein binding to DNA in the presence of water-mediated hydrogen bonds. J. Mol. Model. 5, 125-133 (1999).

Tan, X., Suzuki, N., Johnson, F., Grollman, A.P. and Shibutani, S. Mutagenic properties of the 8-amino-2'-deoxyguanosine DNA adduct in mammalian cells. Nucleic Acids Res. 27, 2310-2314 (1999).

Grollman, A.P., Moriya, M., Shibutani, S . and Takeshita, M. Endogenous DNA damage, mutagenesis, and aging. In: Molecular Biology of Aging. Alfred Benzon Symposium 44. Bohr, V.A., Clark, B.F.C., Stevnsner, T. (Eds.), Munksgaard, Copenhagen, pp. 191-201 (1999).

Shibutani, S., Dasaradhi, L., Terashima, I., Banoglu, E., and Duffel, M.W. Alpha-hydroxytamoxifen is a substrate of hydroxysteroid (alcohol) sulfotransferase, resulting in tamoxifen DNA adducts. Cancer Research 58, 647-653 (1998).

Terashima, I., Suzuki, N., Itoh, S., Yoshizawa, I., and Shibutani, S. Mutagenic specificity of model estrogen-DNA adducts in mammalian cells. Biochemistry 37, 8803-8807 (1998).

Terashima, I., Suzuki, N., Dasaradhi, L., Tan, C.K., Downey, K.M., and Shibutani, S. Translesional synthesis on DNA templates containing an estrogen quinone-derived adduct: N2-(2-hydroxyestron-6-yl)-2'-deoxyguanosine and N6-(2-hydroxyestron-6-yl)-2'-deoxyadenosine. Biochemistry 37, 13807-13815 (1998).

Shibutani, S., Shaw, P.M., Suzuki, N., Dasaradhi, L., Duffel, M.W., and Terashima, I. Sulfation of alpha-hydroxytamoxifen catalyzed by human hydroxysteroid sulfotransferase results in tamoxifen-DNA adducts. Carcinogenesis 19, 2007-2011 (1998).

Itoh, S., Hirai, T., Totsuka, Y., Takagi, H., Tashiro, Y., Wada, K., Wakabayashi, K., Shibutani, S., and Yoshizawa, I. Identification of estrogen-modified nucleosides from calf thymus DNA reacted with 6-hydroxyestrogen 6-sulfates. Chem. Res. Toxicol. 11, 1312-1318 (1998).

Shibutani, S., Fernandes, A., Suzuki, N., Zhou, L., Johnson, F. and Grollman, A.P. Site-specific mutagenesis of the N-(deoxyguanosin-8-yl)-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine DNA adduct in mammalian cells. Z. Lebensm Unters Forsch A 207, 459-463 (1998).

Gelfand, C.A., Plum, G.E., Grollman, A.P., Johnson, F. and Breslauer, K.J. Thermodynamic consequences of an abasic leslion in duplex DNA are strongly dependent on base sequence. Biochemistry 37, 7321-7327 (1998).

Gelfand, C.A., Plum, G.E., Grollman, A.P., Johnson, F. and Breslauer, K.J. The impact of an exocyclic cytosine adduct on DNA duplex properties: Signficant thermodynamic consequences despite modest lesion-induced structural alterations. Biochemistry 37, 12507-12512 (1998).

Lin, Z., Hung, K.-N., Grollman, A.P. and de los Santos, C. Solution structure of duplex DNA containing an extrahelical abasic site analog determined by NMR spectroscopy and molecular dynamics. Nucleic Acids Res. 26, 2385-2391 (1998).

Terasmina, I., Suzuki, N., Dasaradhi, L., Tan, C.K., Downey, K.M., and Shibutani, S. Translesional synthesis on DNA templates containing an estrogen quinone-derived adduct: N2-(2-hydroxyestron-6-yl)-2'-deoxyadenosine. Biochemistry 37, 13807-13815 (1998)

Zharkov, D.O. and Grollman, A.P. MutY DNA glycosylase: base release and intermediate complex formation. Biochemistry 37, 12384-12394 (1998).

Shibutani, S., Suzuki, N. and Grollman, A.P. Mutagenic specificity of acetylaminofluorene-derived DNA adducts in mammalian cells. Biochemistry 37, 12034-12041 (1998).)

Fernandes, A., Liu, T., Amin, S., Geacintov, N.E., Grollman, A.P. and Moriya, M. Mutagenic potential of stereo- isomeric bay region (+)- and (B)-cis-anti-benzo[a]pyrene diol expoxide-N2-deoxyguanosine adducts in Eschericia coli and simian kidney cells. Biochemistry 37, 10164-10172 (1998).

Gening, L., Takeshita, M., Levine, R.L., Peden, K.W. and Grollman, A.P. Extrachromosomal unequal homologus recombination and gene conversion in simian kidney cells: Effects of UVdamage. Mutation Res. 407, 11-24 (1998).

Shibutani, S., Itoh, S., and Yoshizawa, I. Miscoding properties of model estrogen-DNA adducts in reactions catalyzed by mammalian and Escherichia coli DNA polymerases. Biochemistry 36, 1755-1765 (1997).

Dasaradhi, L. and Shibutani, S. Identification of tamoxifen-DNA adducts formed by _-sulfate tamoxifen and _-acetoxytamoxifen. Chem. Res. Toxicol. 10, 189-196 (1997).

Mozzherin, D.J., Shibutani, S., Tan, C.K., Downey, K.M., and Fisher, P.A. Proliferating cell nuclear antigen promotes DNA synthesis past template lesions by mammalian DNA polymerase . Proc. Natl. Acad. Sci. USA 94, 6126-6131 (1997).

Kohda, K., Tsunomoto, H., Kasamatsu, T., Sawamura, F., Terashima, I., and Shibutani, S. Synthesis and miscoding specificity of oligodeoxynucleotide containing 8-phenyl-2'-deoxyguanosine. Chem. Res. Toxicol 10, 1351-1358 (1997).

Shibutani, S. and Dasaradhi, L. Miscoding potential of tamoxifen-derived DNA adducts: _-(N2-deoxy guanosinyl)tamoxifen. Biochemistry 36, 13010-13017 (1997).

Miller, H. and Grollman, A.P. Kinetics of DNA polymerase I (Klenow fragment exo-) activity on damaged DNA templates: Effect of proximal and distal template damage on DNA synthesis. Biochemistry 36, 15336-15342 (1997).

Rosenquist, T., Zharkov, D.O. and Grollman, A.P. Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase. Proc. Natl. Acad. Sci. USA 94, 7429-7434 (1997).PDF(374Kb)

Shibutani, S. and Grollman, A.P. Molecular mechanisms of mutagenesis by aromatic amines and amides. Mutation Res. 376, 71-78 (1997).

Shibutani, S., Takeshita, M., and Grollman, A.P. Translesional synthesis on DNA templates containing a single abasic site: A mechanistic study of the "A rule". J. Biol. Chem. 272, 13916-13922 (1997).

Zharkov, D., Rieger, R.A., Iden, C.R. and Grollman, A.P. NH2-terminal proline acts as a nucleophile in the glycocylase/AP lyase reaction catalyzed by Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg) protein. J. Biol. Chem. 272, 5335-5341 (1997).

Cullinan, D., Johnson, F., Grollman, A.P., Eisenberg, M. and de los Santos, C. Solution structure of a DNA duplex containing the exocyclic lesion 3,N4-etheno-2'-deoxycytidine opposite 2-deoxyguanosine. Biochemistry 36, 11933-11943 (1997).

Karakaya, A., Jaruga, P., Bohr, V.A., Grollman, A.P., and Dizdaroglu, M. Kinetics of excision of purine lesions from DNA by fpg protein. Nucleic Acids Res. 25, 474-479 (1997).

Mozzherin, D. Ju, Shibutani, S., Tan, C-K., Downey, K.M., and Fisher, P.A. Proliferating cell nuclear antigen promotes DNA synthesis past template lesions by mammalian DNA polymerase d. Proc. Natl. Acad. Sci. USA 94, 6126-6131 (1997)

Chen, H., Johnson, F., Grollman, A.P., and Patel, D.J. Structural studies of the ionizing radiation adduct 7,8-dihydro-8-oxoadenine (Aoxo) positioned opposite thymine in a DNA duplex. Magnetic Resonance in Chemistry 34, S23-S32 (1996).

Shibutani, S., Suzuki, N., Matsumoto, Y. and Grollman, A.P. Miscoding properties of 3,N4-etheno-2'-deoxycytidine in reactions catalyzed by mammalian DNA polymerases. Biochemistry 35, 14992-14998 (1996).

Grollman, A.P. and Takeshita, M. 8-Oxoguanine and bistrand abasic sites in DNA: Mutagenic potential and repair. In: 1895-1995 Congress Proceedings Vol. 2. Hagen, U., Harder, D., Jung, H., and Streffer, C. (Eds.), pp. 316-319 (1996).

Greenblatt, M.S., Grollman, A.P., and Harris, C.C. Deletions and insertions of p53 tumor suppressor gene in human cancers: Confirmation of the DNA polymerase slippage/misalignment model. Cancer Res. 56, 2130-2136 (1996).

Cullinan, D., Korobka, A., Grollman, A.P., Patel, D.J., Eisenberg, M., and de los Santos, C. NMR solution structure of an oligodeoxynucleotide duplex containing the exocyclic lesion 3,N4-ethenodeoxycytosine opposite thymine: comparison with the duplex containing deoxyadenosine opposite the adduct. Biochemistry 35, 13319-13327 (1996).

Korobka, A., Cullinan, D., Cosman, M., Grollman, A.P., Patel, D.J., Eisenberg, M., and de los Santos, C. Solution structure of an oligodeoxynucleotide duplex containing the exocyclic lesion 3,N4-ethenodeoxycytosine opposite deoxyadenine, determined by NMR spectroscopy and restrained molecular dynamics. Biochemistry 35, 13310-13318 (1996).

Gelfand, C.A., Plum, G.E., Grollman, A.P., Johnson, F., and Breslauer, K.J. The impact of a bistrand abasic lesion on DNA duplex properties. Biopolymers 38, 439-445 (1996).

Bulychev, N.V., Varaprasad, C.V., Dorman, G., Miller, J.H., Eisenberg, M., Grollman, A.P. and Johnson, F. Substrate specificity of E. coli muty protein. Biochemistry 35, 13147-13156 (1996).

Campbell, G., Deng, Y., Glimm, J., Yu, Q., Eisenberg, M. and Grollman, A.P. Analysis and prediction of protein binding on DNA: Pattern recognition of hydrogen bonds. J. Computational Chemistry 17, 1712-1725 (1996).

Moriya, M., Spiegel, S., Fernandes, A., Amin, S., Liu, T.-M., Geacintov, N.E. and Grollman, A.P. Fidelity of translesional synthesis past benzo[a]pyrene diol epoxide-2 deoxyguanosine DNA adducts: Marked effects of host cell, sequence context, and chirality. Biochemistry 35, 16646-16651 (1996).

Kohda, K., Tsunomoto, H., Minoura, Y., Tanabe, K., and Shibutani, S. Synthesis, miscoding specificity, and thermodynamic stability of oligodeoxynucleotide containing 8-methyl-2'-deoxyguanosine. Chem. Res. Toxicol. 9, 1278-1284 (1996)

Varaprasad, C.V., Bulychev, N., Grollman, A.P. and Johnson, F. Synthesis of 8-oxo-7,8-dihydro-6-O-methyl-2'-deoxyguanosine and its use as a probe to study DNA-base excision by mutY enzyme. Tetrahedron Letters 37, 9-12 (1996).

1995-1990 Top

LePage, F., Margot, A., Grollman, A.P., Sarasin, A., and Gentil, A. Mutagenicity of a unique 8-oxoguanine in a human Ha-ras sequence in mammalian cells. Carcinogenesis 16, 2779-2784 (1995).

Plum, G.E., Grollman, A.P., Johnson, F. and Breslauer, K.J. Influence of the oxidatively damaged adduct 8-oxodeoxyguanosine on the conformation, energetics, and thermodynamic stability of a DNA duplex. Biochemistry 34, 16148-16160 (1995).

Grollman, A.P. and Takeshita, M. Miscoding and mutagenic properties of 8-oxoguanine and abasic sites: Ubiquitious lesions in damaged DNA. In: Radiation Damage to DNA: Structure/Function Relationship of Early Times. Fuciarelli, A.F. and Zimbrick, J.D. (Eds.), Batelle Press, Columbus, Ohio, pp. 293-304 (1995).

Tchou, J. and Grollman, A.P. The catalytic mechanism of Fpg protein: Evidence for a Schiff base intermediate and amino-terminus localization of the catalytic site. J. Biol. Chem. 270, 11671-11677 (1995).

Wilson, D.M. III, Takeshita, M., Grollman, A.P. and Demple, B. Incision activity of human apurinic endonuclease (Ape) at abasic site analogs in DNA. J. Biol. Chem. 270, 16002-16007 (1995).HTML

Grollman, A.P. 8-Oxoguanine in DNA: Its mutagenic properties and repair. Environ. Mut. Res. Commun. 16, 239-243 (1995).

Zhang, W., Johnson, F., Grollman, A.P. and Shibutani, S. Miscoding by the exocyclic DNA adducts: 3,N4-ethano-2'-deoxycytidine and 3-(2-hydroxyethyl)-2'-deoxyuridine. Chem. Res. Toxicol. 8, 157-163 (1995)

Pinz, K., Shibutani, S., and Bogenhagen, D. Action of mitochondrial DNA polymerase g at sites of base loss or oxidative damage. J. Biol. Chem. 270, 9202-9606 (1995).

Moriya, M., Zhang, W., Johnson, F. and Grollman, A.P. Mutagenic potency of exocyclic DNA adducts: Marked differences between Escherichia coli and simian kidney cells. Proc. Natl. Acad. Sci. USA 91, 11899-11903 (1994).

Shibutani, S. and Grollman, A.P. Miscoding during DNA synthesis on damaged DNA templates catalyzed by extracts prepared from mammalian cells. Cancer Letters 83, 315-322 (1994).


Grollman, A.P., Johnson, F., Tchou, J., and Eisenberg, M. Recognition and repair of 8-oxoguanine and formamidopyrimidine lesions in DNA. In: Annals of New York Academy of Sciences Vol. 726. Wallace, S.S. VanHouten, B., and Kow, Y.W. (Eds.), pp. 208-214 (1994).

Tchou, J., Bodepudi, V., Shibutani, S., Antoshechkin, I., Miller, J., Grollman, A.P. and Johnson, F. Substrate specificity of Fpg protein: Recognition and cleavage of oxidatively damaged DNA. J. Biol. Chem. 269, 15318-15324 (1994).

Kasai, H., Chung, M.H., Yamamoto, F., Ohtsuka, E., Laval, J., Grollman, A.P. and Nishimura, S. Formation, inhibition of formation, and repair of oxidative 8-hydroxyguanine DNA damage (review). Basic Life Sciences 61, 257-262 (1993).

Shibutani, S. and Grollman, A.P. Nucleotide misincorporation of DNA templates containing N-(deoxyguanosin-N2-yl)-2-acetylaminofluorene (dG-N2-AAF). Chem. Res. Toxicol. 6, 819-824 (1993).

Tchou, J., Michaels, M.L., Miller, J.H. and Grollman, A.P. Function of the zinc finger domain in Escherichia coli Fpg protein. J. Biol. Chem. 268, 26738-26744 (1993).

Grollman, A.P. and Shibutani, S. Mutagenic specificity of chemical carcinogens as determined by studies with single DNA adducts. In: DNA Adducts: Identification and Biological Significance. Hemminki, K., Dipple, A., Shuker, D., Kadlubar, F.F., Segerback, D. and Bartsch, H. (Eds.), IARC Scientific Publications No. 125, Lyon, France (1993).

Moriya, M. and Grollman, A.P. Mutations of the mutY gene of Escherichia coli enhance the frequency of targeted G:C_T:A transversions induced by a single 8-oxoguanine residue in single-stranded DNA. Molecular Gen. Genet. 239, 72-76 (1993).

Moriya, M. Single-stranded shuttle phagemid for mutagenesis studies in mammalian cells: 8-oxoguanine in DNA
induces targeted G.C-->T.A transversions in simian kidney cells. Proc. Natl. Acad. Sci. USA 90, 1122-1126 (1993).

Shibutani, S. and Grollman, A.P. On the mechanism of frameshift (deletion) mutagenesis in vitro. J. Biol. Chem. 268, 1703-11710 (1993).

Grollman, A.P. and Moriya, M. Mutagenesis by 8-oxoguanine: An enemy within. Trends in Genetics 9, 246-249 (1993).

Shibutani, S., Margulis, L.A., Geacintov, N.E. and Grollman, A.P. Translesional synthesis on DNA template containing a single stereoisomer of dG-(+)- or dG-(-)-anti-BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene). Biochemistry 32, 7531-7541 (1993).

Tchou, J. and Grollman, A.P. Repair of DNA containing the oxidatively-damaged base, 8-oxoguanine. Mutation Res. 299, 277-287 (1993).

Shibutani, S., Bodepudi, V., Johnson, F. and Grollman, A.P. Translesional synthesis on DNA templates containing 8-oxo-7,8-dihydrodeoxyadenosine. Biochemistry 32, 4615-4621 (1993).

Shibutani, S. Quantitation of base substitutions and deletions induced by chemical mutagens during DNA synthesis in vitro. Chem. Res. Toxicol. 5, 625-629 (1993).

Plum, G.E., Grollman, A.P., Johnson, F. and Breslauer, K.J. Influence of an exocyclic guanine adduct on the thermal stability, conformation, and melting thermodynamics of a DNA duplex. Biochemistry 31, 12096-12102 (1992).

Michaels, M.L., Tchou, J., Grollman, A.P. and Miller, J.H. A repair system for 8-oxo-7,8-dihydrodeoxyguanine (8-hydroxyguanine). Biochemistry 31, 10964-10968 (1992).

Michaels, M.L., Cruz, C., Grollman, A.P. and Miller, J.H. Evidence that MutY and MutM combine to prevent mutations by an oxidatively damaged form of guanine in DNA. Proc. Natl. Acad. Sci. USA 89, 7022-7025 (1992).

Grollman, A.P. Structural basis for the mutagenic specificity of 8-oxoguanine-DNA. In: Proceedings of the Seventh Conversation in Biomolecular Stereodynamics. Sarma, R. (Ed.), Adenine Press (1992).

Bodepudi, V., Shibutani, S., and Johnson, F. Synthesis of 2'-deoxy-7,8-dihydro-8-oxoguanosine and 2'-deoxy-
7,8-dihydro-8-oxoadenosine and their incorporation into oligomeric DNA. Chem. Res. Toxicol. 5, 608-617 (1992).

Kalman, T.I., Marinelli, E.R., Xu, B., Benugopala Reddy, A.R., Johnson, F. and Grollman, A.P. Inhibition of cellular thymidylate synthesis by cytotoxic propenal derivatives of pyrimidine bases and deoxynucleosides. Biochemical Pharmacology 42, 432-437 (1991).

Tchou, J., Kasai, H., Shibutani, S., Chung, M.H., Laval, J., Grollman, A.P., and Nishimura, S. 8-Oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity. Proc. Natl. Acad. Sci. USA 88, 4690-4694 (1991).

Moriya, M., Ou, C., Bodepudi, V., Johnson, F., Takeshita, M. and Grollman, A.P. Site-specific mutagenesis using a gapped duplex vector: A study of in vivo translesion synthesis past 8-oxodeoxyguanine. Mutation Res. 254, 281-288 (1991).

Shibutani, S., Gentles, R., Johnson, F. and Grollman, A.P. Isolation and characterization of oligodeoxynucleotides containing dG-N2-AAF and oxidation products of dG-C8-AF. Carcinogenesis 12, 813-818 (1991).

Kouchakdjian, M., Eisenberg, M., Johnson, F., Grollman, A.P., and Patel, D.J. Structural features of an exocyclic adduct positioned opposite an abasic site in a DNA duplex. Biochemistry 30, 3262-3270 (1991).

Kouchakdjian, M., Bodepudi, V., Shibutani, S., Eisenberg, M., Johnson, F., Grollman, A.P., and Patel, D.J. NMR structural studies of the ionizing radiation adduct 8-oxodeoxyguanosine (8-oxodG) opposite deoxyadenosine in a DNA duplex. 8-OxodG(syn):dA(anti) alignment at lesion site. Biochemistry 30, 1403-1412 (1991).

Shibutani, S., Takeshita, M. and Grollman, A.P. Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG. Nature 349, 431-434 (1991).

Chen, Y.H., Matsumoto, Y., Shibutani, S., and Bogenhagen, D.F. Acetylaminofluorene and aminofluorene adducts inhibit in vitro transcription of a Xenopus 5S RNA gene only when located on the coding strand. Proc. Natl. Acad. Sci. USA 88, 9583-9587 (1991).

Kouchakdjian, M., Eisenberg, M., Live, D., Marinelli, E., Grollman, A.P. and Patel, D.J. NMR studies of an exocyclic 1,N2-propanodeoxyguanosine adduct (X) located opposite deoxyadenosine (A) in DNA duplexes at basic pH: Simultaneous partial intercalation of X and A between stacked bases. Biochemistry 29, 4456-4465 (1990).

Grollman, A.P. Site specific mutagenesis. In: Fifth International Conference on Environmental Mutagens.
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